各学部の研究活動
がん分子病態学部 最近3年間の研究業績

発表論文(2012—2015)

  1. Shiro Koizume, Shin Ito, Yusuke Yoshioka, Tomohiko Kanayama, Yoshiyasu Nakamura, Mitsuyo Yoshihara, Roppei Yamada, Takahiro Ochiya, Wolfram Ruf, Etsuko Miyagi, Fumiki Hirahara, Yohei Miyagi. High-level secretion of tissue factor-rich extracellular vesicles from ovarian cancer cells mediated by filamin-A and protease-activated receptors. Thromb Haemost, in press.
  2. Okayama A, Miyagi Y, Oshita F, Ito H, Nakayama H, Nishi M, Kurata Y, Kimura Y, Ryo A, Hirano H. Identification of tyrosine-phosphorylated proteins upregulated during epithelial-mesenchymal transition induced with TGF-β. J Proteome Res. 2015 Jul 28. [Epub ahead of print]
  3. Koizume S, Miyagi Y. Anti-apoptotic genes are synergistically activated in OVSAYO cells cultured under conditions of serum starvation and hypoxia. Genomics Data 5:129-131, 2015. doi:10.1016/j.gdata.2015.05.029
  4. Chijiwa T, Kawai K, Noguchi A, Sato H, Hayashi A, Cho H, Shiozawa M, Kishida T, Morinaga S, Yokose T, Katayama M, Takenaka N, Suemizu H, Yamada R, Nakamura Y, Takano Y, Miyagi Y*, Nakamura M*. Establishment of patient-derived cancer xenografts in immunodeficient NOG mice. Int J Oncol 47: 61-70, 2015. doi: 10.3892/ijo.2015.2997. *co-corespondence
  5. Kikuchi K, Noguchi A, Kasajima R, Miyagi Y, Hoshino D, Koshikawa N, Kubota A, Yokose T, Takano Y. Tumour Biol. 2015 May 7. [Epub ahead of print]
  6. Koizume S, Ito S, Nakamura Y, Yoshihara M, Furuya M, Yamada R, Miyagi E, Hirahara F, Takano Y, Miyagi Y. Lipid starvation and hypoxia synergistically activate ICAM1 and multiple genes in an Sp1-dependent manner to promote the growth of ovarian cancer. Mol Cancer 14(1):77, 2015. doi: 10.1186/s12943-015-0351-z.
  7. Wada H, Shiozawa M, Katayama K, Okamoto N, Miyagi Y, Rino Y, Masuda M, Akaike M. Systematic review and meta-analysis of histopathological predictive factors for lymph node metastasis in T1 colorectal cancer. J Gastroenterol 50(7): 727-34, 2015. doi: 10.1007/s00535-015-1057-0.
  8. Asano R, Asai-Sato M, Miyagi Y, Mizushima T, Koyama-Sato M, Nagashima Y, Taguri M, Sakakibara H, Hirahara F, Miyagi E. Aberrant expression of erythropoietin in uterine leiomyomas and its implication in tumor growth. Am J Obstet Gynecol 213(2):199.e1-8, 2015. doi: 10.1016/j.ajog.2015.02.016.
  9. Nagata K, Kawakami T, Kurata Y, Kimura Y, Suzuki Y, Nagata T, Sakuma Y, Miyagi Y, Hirano H. Augmentation of multiple protein kinase activities associated with secondary imatinib resistance in gastrointestinal stromal tumors as revealed by quantitative phosphoproteome analysis. J Proteomics 115:132-42, 2015. doi: 10.1016/j.jprot.2014.12.012.
  10. Ono K, Hayashi H, Tateno M, Tanaka R, Suzuki R, Maruyama Y, Miyagi Y, Furuya M. Uterine superficial serous carcinomas and extensive serous endometrial intraepithelial carcinomas: clinicopathological analysis of 6 patients. Int J Clin Exp Pathol 7(11):7979-88, 2014.
  11. Koizume S, Miyagi Y. Breast cancer phenotypes regulated by tissue factor-factor VII pathway: Possible therapeutic targets. World J Clin Oncol 5(5):908-920, 2014. doi: 10.5306/wjco.v5.i5.908.
  12. Lin SJ, Gagnon-Bartsch JA, Tan IB, Earle S, Ruff L, Pettinger K, Ylstra B, van Grieken N, Rha SY, Chung HC, Lee JS, Cheong JH, Noh SH, Aoyama T, Miyagi Y, Tsuburaya A, Yoshikawa T, Ajani JA, Boussioutas A, Yeoh KG, Yong WP, So J, Lee J, Kang WK, Kim S, Kameda Y, Arai T, Zur Hausen A, Speed TP, Grabsch HI, Tan P. Signatures of tumour immunity distinguish Asian and non-Asian gastric adenocarcinomas. Gut. 2014 Nov 10. pii: gutjnl-2014-308252. doi: 10.1136/gutjnl-2014-308252.
  13. Nishii T, Yokose T, Miyagi Y, Daigo Y, Ito H, Isaka T, Imai K, Murakami S, Kondo T, Saito H, Oshita F, Yamada K, Matsukuma S, Tsuboi M, Nakayama H, Masuda M. Clinicopathological features and EGFR gene mutation status in elderly patients with resected non-small-cell lung cancer. BMC Cancer 14(1):610, 2014. doi:10.1186/1471-2407-14-610.
  14. Noguchi A, Kikuchi K, Zheng H, Takahashi H, Miyagi Y, Aoki I, Takano Y. SIRT1 expression is associated with a poor prognosis, whereas DBC1 is associated with favorable outcomes in gastric cancer. Cancer Med 3(6):1553-61, 2014. doi: 10.1002/cam4.310.
  15. Kim H, Terazono H, Nakamura Y, Sakai K, Hattori A, Odaka M, Girault M, Arao T, Nishio K, Miyagi Y, Yasuda K. Development of On-Chip Multi-Imaging Flow Cytometry for Identification of Imaging Biomarkers of Clustered Circulating Tumor Cells. PLoS One 9(8):e104372, 2014. doi: 10.1371/journal.pone.0104372.
  16. Harada T, Saito H, Karino F, Isaka T, Murakami S, Kondo T, Oshita F, Miyagi Y, Yamada K. Clinical usefulness of testing for UDP glucuronosyltransferase 1 family, polypeptide A1 polymorphism prior to the inititation of irinotecan-based chemotherapy. Mol Clin Oncol 2(5):737-743, 2014.
  17. Okudela K, Suzuki T, Umeda S, Tateishi Y, Mitsui H, Miyagi Y, Ohashi K. A comprehensive search for microRNAs with expression profiles modulated by oncogenic KRAS: Potential involvement of miR-31 in lung carcinogenesis. Oncol Rep 32(4):1374-84, 2014. doi: 10.3892/or.2014.3339.
  18. Okayama A, Miyagi Y, Oshita F, Nishi M, Nakamura Y, Nagashima Y, Akimoto K, Ryo A, Hirano H. Proteomic Analysis of Proteins Related to Prognosis of Lung Adenocarcinoma. J Proteome Res 13(11):4686-94, 2014. doi: 10.1021/pr4012969.
  19. Okudela K, Tateishi Y, Umeda S, Mitsui H, Suzuki T, Saito Y, Woo T, Tajiri M, Masuda M, Miyagi Y, Ohashi K. Allelic Imbalance in the miR-31 Host Gene Locus in Lung Cancer - Its Potential Role in Carcinogenesis. PLoS One 30;9(6):e100581, 2014. doi: 10.1371/journal.pone.0100581.
  20. Kobayashi Y, Takano A, Miyagi Y, Tsuchiya E, Sonoda H, Shimizu T, Okabe H, Tani T, Fujiyama Y, Daigo Y. Cell division cycle-associated protein 1 overexpression is essential for the malignant potential of colorectal cancers. Int J Oncol 44(1):69-77, 2014. doi: 10.3892/ijo.2013.2177.
  21. Suzuki Y, Miyagi Y, Yukawa N, Rino Y, Masuda M. Epigenetic silencing of checkpoint with fork-head associated and ring finger gene expression in esophageal cancer. Oncol Lett 7(1):69-73, 2014. doi:10.3892/ol.2013.1677.
  22. Komiya E, Sato H, Watanabe N, Ise M, Higashi S, Miyagi Y, Miyazaki K. Angiomodulin, a marker of cancer vasculature, is upregulated by vascular endothelial growth factor and increases vascular permeability as a ligand of integrin αvβ3. Cancer Med 3(3):537-49, 2014. doi: 10.1002/cam4.216.
  23. Numata M, Morinaga S, Watanabe T, Tamagawa H, Yamamoto N, Shiozawa M, Nakamura Y, Kameda Y, Okawa S, Rino Y, Akaike M, Masuda M, Miyagi Y. The clinical significance of SWI/SNF complex in pancreatic cancer. Int J Oncol 42(2):403-10, 2013.
  24. Okudela K, Woo T, Mitsui H, Suzuki T, Tajiri M, Sakuma Y, Miyagi Y, Tateishi Y, Umeda S, Masuda M, Ohashi K. Downregulation of ALDH1A1 expression in non-small cell lung carcinomas - its clinicopathologic and biological significance. Int J Clin Exp Pathol 6(1):1-12, 2013.
  25. Noguchi A, Li X, Kubota A, Kikuchi K, Kameda Y, Zheng H, Miyagi Y, Aoki I, Takano Y. SIRT1 expression is associated with good prognosis for head and neck squamous cell carcinoma patients. Oral Surg Oral Med Oral Pathol Oral Radiol 115(3):385-92, 2013.
  26. van Grieken NC, Aoyma T, Chambers TA, Bottomley D, Ward LC, Inam I, Buffart TE, Das K, Lim T, Pang B, Zhang SL, Tan IB, Carvalho B, Heideman DAM, Miyagi Y, Kameda Y, Arai T, Meijer GA, Tsuburaya A, Tan P, Yoshikawa Y, Grabsch HI. KRAS and BRAF mutations are rare and related to DNA mismatch repair deficiency in gastric cancer from the East and the West: Results from a large international multicentre study. British Journal of Cancer 108(7):1495-501, 2013. doi: 10.1038/bjc.2013.109.
  27. Tamagawa H, Oshima T, Numata M, Yamamoto N, Shiozawa M, Morimnaga S, Nakamura Y, Yoshihara M, Sakuma Y, Kameda Y, Akaike M, Yukawa N, Rino Y, Masuda M, Miyagi Y. Global histone modification of H3K27 correlates with the outcomes in patients with metachronous liver metastasis of colorectal cancer. Eur J Surg Oncol 39(6):655-61, 2013. doi:pii: S0748-7983(13)00268-0. 10.1016/j.ejso.2013.02.023.
  28. Enokida Y, Shimizu K, Atsumi J, Lezhava A, Tanaka Y, Kimura Y, Soma T, Hanami T, Kawai Y, Usui K, Okano Y, Kakegawa S, Ogawa H, Miyamae Y, Miyagi Y, Nakayama H, Ishikawa T, Hayashizaki Y, Takeyoshi I. Rapid Detection of SNP (c.309T>G) in the MDM2 Gene by the Duplex SmartAmp Method. PLoS One. 2013;8(4):e60151. doi: 10.1371/journal.pone.0060151.
  29. Tarao K, Ohkawa S, Miyagi Y, Morinaga S, Ohshige K, Yamamoto N, Ueno M, Kobayashi S, Kameda R, Tamai S, Nakamura Y, Miyakawa K, Kameda Y, Okudaira M. Inflammation in background cirrhosis evokes malignant progression in HCC development from HCV-associated liver cirrhosis. Scand J Gastroenterol 248(6): 729-735, 2013. doi: 10.3109/00365521.2013.782064.
  30. Yoshii T, Miyagi Y, Nakamura Y, Kobayashi O, Kameda Y, Ohkawa S. Pilot research for the correlation between the expression pattern of E-cadherin-β-catenin complex and lymph node metastasis in early gastric cancer. Tumori 99(2):234-8, 2013. doi: 10.1700/1283.14198.
  31. Aoyama T, Yoshikawa T, Miyagi Y, Kameda Y, Shirai J, Hayashi T, Cho H, Oshima T, Yukawa N, Rino Y, Masuda M, Tsuburaya A. Human epidermal growth factor receptor 2 (Her-2) and S-1 adjuvant chemotherapy in stage 2/3 gastric cancer patients who underwent D2 gastrectomy. Surg Today 43(12):1390-7, 2013. doi: 10.1007/s00595-013-0544-2.
  32. Wada H, Shiozawa M, Sugano N, Morinaga S, Rino Y, Masuda M, Akaike M, Miyagi Y. Lymphatic invasion identified with D2-40 immunostaining as a risk factor of nodal metastasis in T1 colorectal cancer. Int J Clin Oncol 18(6):1025-31, 2013. doi: 10.1007/s10147-012-0490-9.
  33. Arakawa N, Miyagi E, Nomura A, Morita E, Ino Y, Ohtake N, Miyagi Y, Hirahara F, Hirano H. Secretome-based identification of TFPI2, a novel serum biomarker for detection of ovarian clear cell adenocarcinoma. J Proteome Res 12(10):4340-50, 2013. doi: 10.1021/pr400282j.
  34. Sakuma Y, Matsukuma S, Nakamura Y, Yoshihara M, Koizume S, Sekiguchi H, Saito H, Nakayama H, Kameda Y, Yokose T, Oguni S, Niki T, Miyagi Y. Enhanced autophagy is required for survival in EGFR-independent EGFR-mutant lung adenocarcinoma cells. Lab Invest 93(10):1137-46, 2013. doi:10.1038/labinvest.2013.102.
  35. Okano Y, Nezu U, Enokida Y, Lee MT, Kinoshita H, Lezhava A, Hayashizaki Y, Morita S, Taguri M, Ichikawa Y, Kaneko T, Natsumeda Y, Yokose T, Nakayama H, Miyagi Y, Ishikawa T. SNP (-617C>A) in ARE-Like Loci of the NRF2 Gene: A New Biomarker for Prognosis of Lung Adenocarcinoma in Japanese Non-Smoking Women. PLoS One 8(9):e73794, 2013. doi: 10.1371/journal.pone.0073794.
  36. Noguchi A, Kikuchi K, Ohtsu T, Yoshiwara M, Nakamura Y, Miyagi Y, Zheng H, Takano Y.Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model. Oncol Rep 30(6):2603-8, 2013. doi: 10.3892/or.2013.2782.
  37. Tae Mogami, Naho Yokota, Mikiko Asai-Sato, Roppei Yamada, Shiro Koizume, Yuji Sakuma, Mitsuyo Yoshihara, Yoshiyasu Nakamura, Yasuo Takano, Fumiki Hirahara, Yohei Miyagi, Etsuko Miyagi. Annexin A4 Is Involved in Proliferation, Chemo-Resistance and Migration and Invasion in Ovarian Clear Cell Adenocarcinoma Cells. PLoS ONE. 8(11):e80359, 2013. Doi:10.1371/journal.pone.0080359
  38. Hayashi H, Miyagi Y, Sekiyama A, Yoshida S, Nakao S, Okamoto N, Koganei K, Sugita A.Colorectal small cell carcinoma in ulcerative colitis with identical p53 gene mutation to associated adenocarcinoma and dysplasia. J Crohns Colitis 6(1):112-115, 2012.
  39. Tamagawa H, Oshima T, Shiozawa M, Morinaga S, Nakamura Y, Yoshihara M, Sakuma Y, Kameda Y, Akaike M, Masuda M, Imada T, Miyagi Y. The global histone modification pattern correlates with overall survival in metachronous liver metastasis of colorectal cancer. Oncol Rep 27(3):637-642, 2012.
  40. Tamagawa H, Miyagi Y, Numata M, Yamamoto N, Shiozawa M, Morinaga S, Sekiyama A, Sekiguchi H, Sakuma Y, Kameda Y, Akaike M, Masuda M, Imada T. Comparison of the chemosensitivity of the primary lesion and a pancreatic metastasis of colon cancer: a case report. Anticancer Res 32(4):1457-1461, 2012.
  41. Komiya E, Furuya M, Watanabe N, Miyagi Y, Higashi S, Miyazaki K. Elevated expression of angiomodulin (AGM/IGFBP-rP1) in tumor stroma and its roles in fibroblast activation. Cancer Sci 103(4): 691-699, 2012.
  42. Sakuma Y, Yamazaki Y, Nakamura Y, Yoshihara M, Matsukuma S, Nakayama H, Yokose T, Kameda Y, Koizume S, Miyagi Y. WZ4002, a third-generation EGFR inhibitor, can overcome anoikis resistance in EGFR-mutant lung adenocarcinomas more efficiently than Src inhibitors. Lab Invest 92(3):371-383, 2012.
  43. Endoh K, Nishi M, Ishiguro H, Uemura H, Miyagi Y, Aoki I, Hirano H, Kubota Y, Ryo A. Identification of phosphorylated proteins involved in the oncogenesis of prostate cancer via Pin1-proteomic analysis. Prostate 72(6):626-637, 2012.
  44. Ohe M, Yokose T, Sakuma Y, Miyagi Y, Okamoto N, Osanai S, Hasegawa C, Nakayama H, Kameda Y, Yamada K, Isobe T. Stromal micropapillary component as a novel unfavorable prognostic factor of lung adenocarcinom. Diagn Pathol 7:3, 2012.
  45. Furuya M, Tanaka R, Miyagi E, Kami D, Nagahama K, Miyagi Y, Nagashima Y, Hirahara F, Inayama Y, Aoki I. Impaired CXCL4 expression in tumor-associated macrophages (TAMs) of ovarian cancers arising in endometriosis. Cancer Biol Ther 13(8):671-680, 2012.
  46. Cho H, Watanabe T, Aoyama T, Hayashi T, Yamada T, Ogata T, Yoshikawa T, Tsuburaya A, Sekiguchi H, Nakamura Y, Sakuma Y, Kameda Y, Miyagi Y. Small bud of probable gastrointestinal stromal tumor within a laparoscopically-resected gastric schwannoma. Int J Clin Oncol 17(3):294-298, 2012.
  47. Morinaga S, Nakamura Y, Watanabe T, Mikayama H, Tamagawa H, Yamamoto N, Shiozawa M, Akaike M, Ohkawa S, Kameda Y, Miyagi Y. Immunohistochemical Analysis of Human Equilibrative Nucleoside Transporter-1 (hENT1) Predicts Survival in Resected Pancreatic Cancer Patients Treated with Adjuvant Gemcitabine Monotherapy. Ann Surg Oncol 19(3):558-564, 2012.
  48. Koizume S, Ito S, Miyagi E, Hirahara F, Nakamura Y, Sakuma Y, Osaka H, Takano Y, Ruf W, Miyagi Y. HIF2-Sp1 interaction mediates a deacetylation-dependent FVII gene activation under hypoxic conditions in ovarian cancer cells. Nucleic Acid Res 40(12):5389-5401, 2012.
  49. Fukahori M, Yoshida A, Hayashi H, Yoshihara M, Matsukuma S, Sakuma Y, Koizume S, Okamoto N, Kondo T, Masuda M, Miyagi Y. The association between RAS gene mutations and clinical characteristics in follicular thyroid tumors: new insights from a single center and a large patient cohort. Thyroid 22(7):683-689, 2012.
  50. Sakuma Y, Yamazaki Y, Nakamura Y, Yoshihara M, Matsukuma S, Koizume S, Miyagi Y. NF-κB signaling is activated and confers resistance to apoptosis in three-dimensionally cultured EGFR-mutant lung adenocarcinoma cells. Biochem Biophys Res Commun 423(4):667-671, 2012. (IF=1.8, 2011)
  51. Washimi K, Yokose T, Yamashita M, Kageyama T, Suzuki K, Yoshihara M, Miyagi Y, Hayashi H, Tsuji S. Specific Expression of Human Intelectin-1 in Malignant Pleural Mesothelioma and Gastrointestinal Goblet Cells. PLoS ONE 7(7):e39889. doi:10.1371/journal.pone.0039889, 2012.
  52. Tarao K, Miyakawa K, Miyagi Y, Ohkawa S, Morinaga S, Oshige K, Yamamoto N, Ueno M, Kobayashi S, Kameda R, Tamai S, Nakamura Y, Endo Y, Kameda Y, Okudaira M. Severe Inflammation in the Background Liver Cirrhosis Correlates with the Development of Poorly Differentiated HCC in HCV-Associated Liver Cirrhosis. Intern Med 51(18):2495-501, 2012.
  53. Watanabe T, Morinaga S, Akaike M, Numata M, Tamagawa H, Yamamoto N, Shiozawa M, Ohkawa S, Kameda Y, Nakamura Y, Miyagi Y. The cellular level of histone H3 lysine 4 dimethylation correlates with response to adjuvant gemcitabine in Japanese pancreatic cancer patients treated with surgery. Eur J Surg Oncol 38(11):1051-7, 2012. doi: 10.1016/j.ejso.2012.08.008.

特許出願など(2012—2015)

  1. 発明の名称:上皮間葉系転換に着目した早期肺腺癌悪性度診断マーカーの利用
    出願番号:特願2015−94599
    出願日:平成27年5月7日
    出願人:地方独立行政法人神奈川県立病院機構、公立大学法人横浜市立大学
    発明者:岡山明子、木村弥生、梁明秀、平野久、宮城洋平、中山治彦、伊藤宏之、山田耕三、尾下文浩、横瀬智之.
  2. 発明の名称:大腸がんの転移又は再発リスクの評価方法
    出願番号:特願2014−017945
    出願日:平成26年1月31日
    出願人:学校法人順天堂、地方独立行政法人神奈川県立病院機構、独立行政法人理化学研究所
    発明者:小見山博光、坂本一博、奥澤淳司、塩澤学、赤池信、宮城洋平、大津敬、林崎良英、伊藤昌可、川路英哉、大宮寛子
  3. 発明の名称:乳がんの転移又は再発リスクの評価方法
    出願番号:特願2014−017946
    出願日:平成26年1月31日
    出願人:地方独立行政法人神奈川県立病院機構、学校法人順天堂、独立行政法人理化学研究所
    発明者:清水哲、宮城洋平、大津敬、齋藤光江、林崎良英、伊藤昌可、川路英哉、大宮寛子.

各学部の研究活動